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Appearance : Light blue amorphous powder, lyophilized
Activity : GradeⅢ 200U/mg-solid or more (containing approx. 70% of stabilizers)
Contaminants : Catalase ≤1.0×10-1 % Phosphatase ≤2.0×10-2 %
Stabilizers : Bovine serum albumin (BSA), borax, basic amino acids.
Stability : Store at -20℃ (A decrease in activity of ca.20% may occur at 5℃ within 6 months.)
Molecular weight : Approx. 140,000
Isoelectric point : between 6.0 and 7.8.8 2
Michaelis constant : 2.5×10-4 M (Ascorbate)
Structure : 8 copper atoms per mol of enzyme 1,2
Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na)
Optimum pH : 5.6
Optimum temperature : approx. 30℃
pH Stability : pH 7.0-10.0 (25℃, 17hr)
Thermal stability : below 40℃ (pH 8.0, 30min)
Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.