Cholesterol oxidase

Cholesterol oxidase

Cholesterol oxidase from Microorganism

Cholesterol + O2 ----> Choleste-4-en-3-one + H2O2

This enzyme is used for enzymatic determination cholesterol in serum when coupled with cholesterol esterase in clinical analysis.

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Product name: Cholesterol: oxygen oxidoreductase
Appearance: Yellow to brownish lyophilized powder
Activity: 25 U/mg or more
Contaminants: Catalase: < 1 %
Cholesterol esterase: < 0.1 %
Glucose oxidase: < 0.1 %
Stability: Stable at - 20 °C for at least one year
Stabilizers: Bovine serum albumin, amino acids
Molecular weight: Approx. 56,000 (by gel-filtration)
Michaelis constants: 2 x 10-6M (Cholesterol)
Inhibitors: Ionic detergents, Hg2+
Optimum pH: 6.5 - 8.0
Optimum temperature: 55 °C
pH Stability: pH 4.5 - 10.0 (25°C, 20hr)
Thermal stability: Below 60°C (pH 7.0, 15 min)

EC # 1.1.3.6

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