Glucoamylase from Rhizopus sp.Starch + nH2O ----> β-D-glucoseStarch + nH2O <---- β-D-glucoseThis enzyme is used for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis.
Glucose oxidase from Aspergillus sp.
β-D-glucose + O2 + H2O ----> D-glucono-δ-Lactone + H2O2
β-D-glucose + O2 + H2O <---- D-glucono-δ-Lactone + H2O2
This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH- 211, if maltooligosaccharide or modified starch is used as a substrate) in clinical analysis.