Glucoamylase from Rhizopus sp.Starch + nH2O ----> β-D-glucoseStarch + nH2O <---- β-D-glucoseThis enzyme is used for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis.
Glutamate dehydrogenase (NADP dependent)
Glutamate dehydrogenase (NADP dependent) from Proteus sp.
L-Glutamate + H2O + NADP+ ----> 2-Oxoglutarate + NH3 + NADPH + H+
L-Glutamate + H2O + NADP+ <---- 2-Oxoglutarate + NH3 + NADPH + H+
This enzyme is used for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease.