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Product name Glycerol:NAD+ 2-oxydoreductase
Appearance White amorphous powder lyophilized
Activity Grade III 50 U/mg-solid or more (containing 50% of stabilizers)
Contaminants NADH oxidase ≤ 1.0×10-3 %
Stabilizers BSA
Stability Stable at -20°C for at least six month
Molecular weight approx. 390000
Isoelectric point 4.4±0.1
Michaelis constants 1.1×10-2M (Glycerol) 8.9×10-5M (NAD+)
Structure 10 subunits (42000) per mol of enzyme
Inhibitors p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions
(Co2+, Ni2+, Cu2+, Zn2+, Cd2+)
Optimum pH 10.0-10.5
Optimum temperature 50°C
pH Stability pH 7.5-10.5 (25°C, 20hr)
Thermal stability below 55°C (pH 7.5, 15min)
Substrate specificity This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also
oxidizes glycerol-α-monochlorohydrin, ethylene glycol and 2,3-butanediol (Table 1).
The oxidative reaction is stimulated by K+, NH4+ and Rb+.
EC # 1.1.1.6