This enzyme is used for enzymatic determination of glycerol and triglyceride
when coupled with glycerol-3-phosphate dehydrogenase G-3-P DH (G3D-301), glycerol-3-phosphate oxidase G-3-P oxidase (G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.
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Product name ATP: Glycerol 3-phosphotransferase
Appearance White amorphous powder lyophilized
Activity Grade III 20 U/mg-solid or more (containing approx. 50% stabilizers)
Contaminants NADH oxidase ≤ 1.0×10-1 %
Catalase ≤ 1.0×10-2 %
Phosphatase (pH 6) ≤ 1.0×10-3 %
Stability Stable at - 20℃ for at least one year
Molecular weight approx. 128,000 (by gel filtration)
Isoelectric point 4.2
Michaelis constants 4.4×10-5 M (Glycerol), 4.3×10-4M (ATP)
Inhibitors p-Chloromercuribenzoate, heavy metal ions (Pb2＋, Fe2+, Hg2+, Ag＋)
Optimum pH 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system)
Optimum temperature 50 °C
pH Stability pH 5.5 - 10.0 (25 °C, 20hr)
Thermal stability below 40 °C (pH 7.5, 15min)
Substrate specificity This enzyme catalyzes the stereospecific transfer of the terminal phosphoryl
moiety of ATP to one of the primary hydroxyl group of glycerol, forming snglycerol-
3-P. The enzyme has the highest specificity for glycerol, and also
phosphorylates dihydroxyacetone and glyceraldehyde. Mg2+ is essentially
required for the reaction.
EC # 188.8.131.52