Glucoamylase from Rhizopus sp.Starch + nH2O ----> β-D-glucoseStarch + nH2O <---- β-D-glucoseThis enzyme is used for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis.
D-3-Hydroxybutyrate dehydrogenase from Pseudomonas sp.
D-3-hydroxybutyrate + NAD + ----> Acetoacetate + NADH + H+
D-3-hydroxybutyrate + NAD + <---- Acetoacetate + NADH + H+
This enzyme is used for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.