Glucoamylase from Rhizopus sp.Starch + nH2O ----> β-D-glucoseStarch + nH2O <---- β-D-glucoseThis enzyme is used for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis.
L-Lactate dehydrogenase from Pig heart
L-Lactate + NAD+ ---> Pyruvate + NADH + H+
L-Lactate + NAD+ <--- Pyruvate + NADH + H+
This enzyme is used for enzymatic determination of numerous metabolites, e.g.ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g.GPT, PK and CPK when coupled with the related enzymes.