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Product name L-Leucine: NAD+ oxidoreductase (deaminating)
Appearance White amorphous powder lyophilized
Activity GradeⅡ 20U/mg-solid or more (containing approx. 70% of
stabilizers)
Contaminants Leucylpeptide decomposing enzymes :
(Leu-Val) ≤ 1.0×10-2%
(Leu-Gly-Gly) ≤ 1.0×10-2%
NADH oxidase ≤ 1.0×10-2%
Stabilizers 2-Mercaptoethanol, L-cysteine, dithiothreitol,
ethylenediaminetetraacetate
Stability Stable at -20℃ for at least one year
Molecular weight 245,000
Michaelis constants 1.0×10-3M (L-Leucine), 3.9×10-4M (NAD+), 3.5×10-5M
(NADH), 3.1×10-4M[α-Ketoisocaproate (α-KⅠC)],
2.0×10-1M (NH3)
Structure 6 subunits per mol of enzyme
Inhibitors Na2S, Hg2+, Cu2+, Co2+, Mg2+, p-chloromercuribenzoate
Optimum pH 10.5-10.8 (L-Leu→α-KⅠC), 9.4 (α-KⅠC→L-Leu )
Optimum temperature Above 70℃
pH Stability pH 5.5-10.5 (25℃, 20hr)
Thermal stability Below 60℃ (pH 6.9, 10min)
EC # 1.4.1.9