Glucoamylase from Rhizopus sp.Starch + nH2O ----> β-D-glucoseStarch + nH2O <---- β-D-glucoseThis enzyme is used for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis.
Leucine dehydrogenase from Bacillus sp.
L-Leucine + H2O + NAD+ ----> α-Ketoisocaproate + NH3 + NADH + H+
L-Leucine + H2O + NAD+ <---- α-Ketoisocaproate + NH3 + NADH + H+
This enzyme is used for enzyme determination of L-leucine and the activity of leucine aminopeptidase.