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Leucine dehydrogenase
Leucine dehydrogenase from Bacillus sp.
L-Leucine + H2O + NAD+ ----> α-Ketoisocaproate + NH3 + NADH + H+
L-Leucine + H2O + NAD+ <---- α-Ketoisocaproate + NH3 + NADH + H+
This enzyme is used for enzyme determination of L-leucine and the activity of leucine aminopeptidase.
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Leucine dehydrogenase from Bacillus sp.
L-Leucine + H2O + NAD+ ----> α-Ketoisocaproate + NH3 + NADH + H+
L-Leucine + H2O + NAD+ <---- α-Ketoisocaproate + NH3 + NADH + H+
This enzyme is used for enzyme determination of L-leucine and the activity of leucine aminopeptidase.
LED-201
Glucoamylase from Rhizopus sp.Starch + nH2O ----> β-D-glucoseStarch + nH2O <---- β-D-glucoseThis enzyme is used for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis.
Glucose-dehydrogenase (NADP(P)-dependent) from Microorganismβ-D-Glucose + NAD(P)+ ----> D-glucono-δ-Lactone + NAD(P)H + H+This enzyme is used for enzymatic determination of D-GlucoseIf you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Glucose-dehydrogenase (PQQ-dependent) from MicroorganismD-Glucose + Acceptor (ox) ----> D-glucono-1,5-Lactone + Acceptor (red)This enzyme is used for enzymatic determination of D-Glucose
Glucose-dehydrogenase (FAD-dependent) from MicroorganismD-Glucose:(flavine adenine dinucleotide)-dehydrogenaseEC # 1.1.99.10If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Glucose oxidase from Aspergillus sp.β-D-glucose + O2 + H2O ----> D-glucono-δ-Lactone + H2O2β-D-glucose + O2 + H2O <---- D-glucono-δ-Lactone + H2O2This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH- 211, if maltooligosaccharide or modified starch is used as a...
Glucose oxidase from Aspergillus sp.β-D-glucose + O2 + H2O ----> D-glucono-δ-Lactone + H2O2β-D-glucose + O2 + H2O <---- D-glucono-δ-Lactone + H2O2This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH- 211, if maltooligosaccharide or modified starch is used as a...
Glucose oxidase from Aspergillus sp.β-D-glucose + O2 + H2O ----> D-glucono-δ-Lactone + H2O2β-D-glucose + O2 + H2O <---- D-glucono-δ-Lactone + H2O2This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH-211, if maltooligosaccharide or modified starch is used as a...
Glutaminase from E. coliL-Glutamine amidohydrolaseEC # 3.5.1.2If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Glutamic-oxaloacetic transaminase from Porcine heartThis enzyme is used in multi analyte clinical chemistry controls and calibrators.If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Glutamic-pyruvate transaminase from Porcine heartThis enzyme is used in multi analyte clinical chemistry controls and calibrators.If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Glutamate dehydrogenase from Beef liver — GTD-07C (EC 1.4.1.3)L-Glutamate + H2O + NADP+ ----> 2-Oxoglutarate + NH3 + NADPH + H+L-Glutamate + H2O + NADP+ <---- 2-Oxoglutarate + NH3 + NADPH + H+This enzyme is used for enzymatic determination of NH3, α-ketoglutaric acid and Lglutamic acid, and for assay of leucine aminopeptidase and urease...
Glutamate dehydrogenase (NADP dependent) from Proteus sp.L-Glutamate + H2O + NADP+ ----> 2-Oxoglutarate + NH3 + NADPH + H+L-Glutamate + H2O + NADP+ <---- 2-Oxoglutarate + NH3 + NADPH + H+This enzyme is used for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease.
Glutamate dehydrogenase (NAD dependent) from MicroorganismL-Glutamate + H2O + NADP+ ----> 2-Oxoglutarate + NH3 + NADPH + H+L-Glutamate + H2O + NADP+ <---- 2-Oxoglutarate + NH3 + NADPH + H+This enzyme is used for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease.
L-Glutamate oxidase from Streptomyces sp. L-Glutamate:oxygen oxidoreductase (deaminating)EC # 1.4.3.11If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Glycerol dehydrogenase from Cellulomonas sp.Glycerol + NAD+ ----> Dihydroxiacetone + NADH + H+Glycerol + NAD+ <---- Dihydroxiacetone + NADH + H+This enzyme is used for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.
Glycerol kinase from Cellulomonas sp.Glycerol + ATP ----> Glycerol-3-phosphate + ADPMg2+Glycerol + ATP <---- Glycerol-3-phosphate + ADPMg2+This enzyme is used for enzymatic determination of glycerol and triglyceridewhen coupled with...
Glycerol kinase from MicroorganismGlycerol + ATP ----> Glycerol-3-phosphate + ADPMg2+Glycerol + ATP <---- Glycerol-3-phosphate + ADPMg2+ This enzyme is used for enzymatic determination of glycerol and triglyceride when coupled with...
D-3-Hydroxybutyrate dehydrogenase from Pseudomonas sp.D-3-hydroxybutyrate + NAD + ----> Acetoacetate + NADH + H+D-3-hydroxybutyrate + NAD + <---- Acetoacetate + NADH + H+This enzyme is used for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.
p-Hydroxybenzoate hydroxylase from Microorganism4-hydroxybenzoate, NADPH:oxygen oxidoreductase (3-hydroxylating)EC # 1.14.13.2If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Hexokinase from MicroorganismD-hexose + ATP ----> D-Hexose-6-Phosphate + ADP+ Mg2+D-hexose + ATP <---- D-Hexose-6-Phosphate + ADP+ Mg2+ This enzyme is used for enzymatic determination of glucose and adenosine-5'-triphosphate (ATP) when coupled with glucose-6-phosphate ehydrogenase G-6-PDH (G6D-311, G6D-321).
Lactate oxidase from MicroorganismL-Lactate + O2 ----> Pyruvate + H2O2L-Lactate + O2 <---- Pyruvate + H2O2This enzyme is used for enzymatic determination of L-Lactate when coupled with the related enzymes.If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
L-Lactate dehydrogenase from Pig heartL-Lactate + NAD+ ---> Pyruvate + NADH + H+L-Lactate + NAD+ <--- Pyruvate + NADH + H+This enzyme is used for enzymatic determination of numerous metabolites, e.g.ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g.GPT, PK and CPK when coupled with the related enzymes.
D-Lactate dehydrogenase from MicroorganismD-Lactate + NAD+ ---> Pyruvate + NADH + H+D-Lactate + NAD+ <--- Pyruvate + NADH + H+This enzyme is used for enzymatic determination of numerous metabolites, e.g.ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g.GPT, PK and CPK when coupled with the related enzymes.
Lactate dehydrogenase from Rabbit muscle/Porcine heart(S)-Lactate: NAD+ oxidoreductaseEC # 1.1.1.27If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Lactate oxidase from MicroorganismL-Lactate + O2 ----> Pyruvate + H2O2L-Lactate + O2 <---- Pyruvate + H2O2This enzyme is used for enzymatic determination of L-lactate.If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Lipoproteine lipase from Pseudomonas sp.Triglyceride + 3H2O ----> Glycerol +3 fatty acidThis enzyme is used for enzymatic determination of triglyceride in serum when coupled with...
Lipoproteine lipase from MicroorganismTriglyceride + 3H2O ----> Glycerol +3 Fatty acidThis enzyme is used for enzymatic determination of triglyceride in serum when coupled with...
Lipoproteine lipase "chemically stabilized" from MicroorganismEC # 3.1.1.34If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Alkaline phosphatase from Calf intestineOrthophosphoric monoester + H2O ----> Alcohol + OrthophosphateThis enzyme is used in molecular biology and in immuo-assays.If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Product name L-Leucine: NAD+ oxidoreductase (deaminating)
Appearance White amorphous powder lyophilized
Activity GradeⅡ 20U/mg-solid or more (containing approx. 70% of
stabilizers)
Contaminants Leucylpeptide decomposing enzymes :
(Leu-Val) ≤ 1.0×10-2%
(Leu-Gly-Gly) ≤ 1.0×10-2%
NADH oxidase ≤ 1.0×10-2%
Stabilizers 2-Mercaptoethanol, L-cysteine, dithiothreitol,
ethylenediaminetetraacetate
Stability Stable at －20℃ for at least one year
Molecular weight 245,000
Michaelis constants 1.0×10-3M (L-Leucine), 3.9×10-4M (NAD＋), 3.5×10-5M
(NADH), 3.1×10-4M［α-Ketoisocaproate (α-KⅠC)］,
2.0×10-1M (NH3)
Structure 6 subunits per mol of enzyme
Inhibitors Na2S, Hg2+, Cu2+, Co2+, Mg2+, p-chloromercuribenzoate
Optimum pH 10.5－10.8 (L-Leu→α-KⅠC), 9.4 (α-KⅠC→L-Leu )
Optimum temperature Above 70℃
pH Stability pH 5.5－10.5 (25℃, 20hr)
Thermal stability Below 60℃ (pH 6.9, 10min)
EC # 1.4.1.9
