This enzyme is used for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5~3.0 units of the enzyme per test (3.0ml) at pH around 7.0.
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Product name Triacylglycero-protein acylhydrolase
Appearance Light brown amorphous powder lyophilized
Activity Grade III 20U/mg-solid or more (containing approx. 80%
of stabilizers)
Contaminants NADH oxidase ≤ 1.0×10-3 %
Phosphatase ≤ 1.0×10-3 %
Catalase ≤ 2.0×10-2%
Cholesterol oxidase ≤ 2.0×10-3 %
Stabilizers Mg2+, Na-cholate, bovine serum albumin
Stability Stable at -20°C for at least one year
Molecular weight approx. 134,000
Isoelectric point 5.95 ± 0.05
Inhibitors Hg2+, Ag+, ionic detergents
Optimum pH 7.0-9.0
Optimum temperature 45-50°C
pH Stability pH 7.0-9.0 (25°C, 20hr)
Thermal stability below 55°C (pH 7.0, 10min)
EC # 3.1.1.34