Phospholipase D

Phospholipase D

Phospholipase D from Streptomyces chromofuscus

Phosphatidylcholine + H2O ---> Phosphatidic acid + Choline

This enzyme is used for enzymatic determination of phospholipids in clinical analysis.

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Name: Phosphatidylcholine phosphatidohydrolase
Appearance: Brown powder lyophilized
Activity: 40U/mg-solid or more
Specific activity: 150U/mg protein or more
Stability: Stable at -20°C for at least 12 months
Molecular weight: approx. 57 000 by SDS electrophoresis
Isoelectric point: 5.1
Michaelis constant: 1.4×10-3 M (phosphatidylcholine)
Inhibitors: EDTA
Optimum: pH 7.0—8.5
pH stability: 6.0—10.0
Thermal Stability: Stable at below 60°C (pH 8.0, 10min)
Activators: Ca, Triton X-100, DOC, Adekatol S0-120
Contaminating: enzymes Catalase : < 0.6 % - Glucose oxidase : < 0.02 %

EC # 3.1.4.4

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