Reference: PYK-309P

Pyruvate kinase from Microorganism

Pyruvate kinase from Microorganism ATP + Pyruvate ---> Phosphoenolpyruvate + ADP ATP + Pyruvate <--- Phosphoenolpyruvate + ADP This enzyme is used for enzymatic determination of ADP, ATP, pyruvate or phosphoenolpyruvate(PEP) when coupled with the related enzymes.

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Product name : ATP : pyruvate 2-0-phosphotransferase
EC # 2.7.1.40
Appearance : White amorphous powder, lyophilized
Activity : ≥ 25 U/mg powder
NADH oxidase : ≤ 0.01 %
Stability : Stable at - 20℃ for at least 12 months
Molecular weight : 63 kDa (SDS-PAGE)
Isoelectric point : 5.7
Michaelis constant : 8.7×10^-4 M (ADP), 4.5×10^-4 M (PEP)
Inhibitors : Not inhibited by NaN3
Optimum pH : 6.2
Optimum temperature : 50 °C
pH Stability : pH 5.5 - 9.0 (25 °C, 16 h)
Thermal stability : Below 60 °C (pH 7.5, 30 min)

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Pyruvate kinase from Microorganism

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